DNA Vectors
Vector Biolabs AAV-CreERT2 (AAV serotype DJ) AAV (AAVDJ-CreERT2)
A mutated form of estrogen ligand-binding domain (ERT2) that binds to synthetic antagonists (such as tamoxifen or its derivative 4-hydroxy-tamoxifen) but not to circulating estrogens was fused to the Cre recombinase (Cre) to create the CreERT2, which requires the presence of tamoxifen for Cre recombinase activity. Therefore it makes possible to induce the recombination in cells with tamoxifen.The AAV-DJ is a synthetic serotype made from DNA family shuffling of 8 wild type serotypes of AAV, including AAV2, 4, 5, 8, 9, avian, bovine and goat AAV. AAV-DJ outperformed standard AAV serotype 1-8 in cultured cells.This AAV/DJ virus expresses CreERT2 under a CMV promoter.
Vector Biolabs human IKKa shRNA Adenovirus (Ad-h-IKKa-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human IKKa gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by western blot in A549 cells.
Vector Biolabs FLAT tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-GFP-FLAG-hCas9)
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized spCas9 nuclease with FLAG tag and a GFP reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc.
Vector Biolabs Cre Inducible (FlexON) GCaMP6m Adenovirus (Ad-DIO-GCaMP6m)
GCaMP is a genetically encoded calcium indicator (GECI) which was created from a fusion of green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. GECI binds Ca2+ and induce a change in fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ fluxes. The advantage of GECI's are that they can be genetically specified for studies in living organisms.The new novel GCaMP6 have increased ΔF/F0 and faster kinetics compared to previous GCaMP3 and GCaMP5G.This adenovirus expresses GCaMP6m in a FLEX system for Cre inducible expression.
Vector Biolabs AAV-RFP-iCre (AAV9) AAV (AAV9-RFP-iCre)
Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By applying the mammalian codon usage to Cre recombinase, expression of Cre is improved in the mammalian cells. This improved Cre (iCre) gene also reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals.This AAV-DJ/9 stock expresses an improved Cre (iCre) and a RFP driven by one CMV promoter. The iCre and RFP was separated by a 2A peptides.
Vector Biolabs Protein kinase C, gamma Adenovirus (Ad-PKC-gamma)
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth, differentiation, gene expression, and hormone action. PKCs are protein serine/threonine kinases whose activities are dependent on calcium and phospholipids. PKCs can be subdivided into two major classes, including conventional (c) PKC isoforms and novel (n) isoforms. A more recently described member of this family has been designated either PKC µ for the human protein or PKD for isolates of mouse origin.
Vector Biolabs Baculoviral IAP repeat-containing 3 Adenovirus (Ad-BIRC3)
TRAF-interacting proteins include TANK (also designated I-TRAF), ILP, ML-IAP, XAF1, c-IAP and survivin. TANK binds to the conserved TRAF-C domain common to TRAF1, TRAF2 and TRAF3. ML-IAP, c-IAP1 and c-IAP2 are related to the inhibitor of apoptosis protein (IAP) family of Baculovirus proteins. The c-IAPs associate with TRAF1 and TRAF2 through their N-terminal BIR motif-containing domain. IAP-like protein (ILP) also contains an N-terminal BIR motif-containing domain. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. An additional IAP family member, survivin (also designated TIAP), is expressed in the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindles. Caspase-3 activity increases when a disruption of survivin-microtubule interactions occurs.
Vector Biolabs Phosphodiesterase 1C, calmodulin-dependent 70kDa Adenovirus (Ad-PDE1C)
The cyclic nucleotide phosphodiesterases (PDEs) have an important role to play in the regulation of cyclic nucleotide levels, as PDE activity hydrolyses camp and cGMP [1-5]. The PDEs are a large superfamily of enzymes consisting of eleven discovered isozyme families (PDE1-11) that differ in respect to substrate specificity, subcellular distribution and regulatory properties [2, 3, 6-9]. Of these eleven families, cGMP-activated PDE (PDE3), cAMP-specific PDE (PDE4), and the more recently discovered PDE7 and PDE8 isozymes mainly hydrolyse cAMP. The PDE5, PDE6 and PDE9 isozymes prefer cGMP as a substrate. Calcium/calmodulin-stimulated PDE (PDE1), cGMP-stimulated PDE (PDE2), PDE10 and PDE11 are dual-substrate PDEs that hydrolyse both cAMP and cGMP.
Agilent Technologies PSHUTTLE-CMV LACZ VECTOR
PShuttle-CMV lacZ Vector is a control vector produced by insertion of the lacZ gene in the multiple cloning site of the pShuttle-CMV. This construct is provided as a control for the production of recombinant adenovirus
BIOHIPPO Empty Vector CMV-Flag/BFP/BSD
Empty Vector CMV-Flag/BFP/BSD lentiviral vector expressing BFP under CMV promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Lentiviral particles packaged with Empty Vector Ctrl ORF cDNA Lentivirus CMV promoter drives expression of fluorescent reporter and resistance marker separated by P2A An epitope tag remains upstream of the fluorescent reporter separated by T2A and would otherwise be C-terminal to the ORF cDNA which is not present in this empty vector control This product is great for use as a negative control for any of our ORF cDNA lentiviral particles You can choose the empty vector construct containing the epitope tag reporter and selection marker to match the ORF cDNA lentivirus product you are using The transduced cells will express all these components without any ORF cDNA insert Vector Layout 5 LTR-CMV-[no cDNA]-tag-T2A-Reporter-P2A-Selection-3 LTR
OriGene PCMV6-AN-GFP, MAMMALIAN VECTOR
Pcmv6-An-Gfp, Mammalian Vector With N-Terminal Tgfp Tag, 10Ug
Vector Biolabs Fas Ligand (tet-on) Adenovirus (Ad-Tet-on-FasL)
The FasL transgene is under the control of a tetracycline-regulated promoter.
Vector Biolabs human STK16 shRNA Adenovirus (Ad-h-STK16-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human STK16 gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by qPCR in A549 cells.